1) A mutation in the Lac Repressor (LacI) promoter that prevents Sigma Factor from binding would have the same phenotype as which of the following? a.An IS mutation b.A mutation in the Lac Operon CAP

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1) A mutation in the Lac Repressor (LacI) promoter that prevents Sigma Factor from binding would have the same phenotype as which of the following?

a.An IS mutation

b.A mutation in the Lac Operon CAP binding site that prevents CAP from binding

c. An OC mutation

d. The presence of glucose in the media

e. A Lac Operon P- mutation

2) What is missing from elongating RNA polymerase? Sometimes called the ternary complex?

a. Beta’ (Beta prime) subunit

b. All of these are part of the ternary complex

c. Sigma Factor

d. Beta subunit

e. Omega subunit

3) You mutagenize cells with 5-BU that causes transition mutations. Which of the following amino acid replacements is most likely?

a. Met (AUG) -> Leu (UUG)

b. Trp (UGG) -> Gly (GGG)c. Met (AUG) -> Lys (AAG)

d. Trp (UGG) -> Cys (UGC)

e. Met (AUG) -> Thr (ACG)

4) For each of the following genotypes, select the color of resulting colonies when plated on X-gal + Lactose media. Choose Blue or White

a) I+P+O+Z-Y+b) I-P+O+Z+Y- / I+P+OcZ-Y+c) I+P+O+Z+Y+d) I+P+O+Zalpha-Y+

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